Oncomodulin, also known as OCM, is an oncodevelopmental protein found in early embryonic cells in the placenta and also in tumors. The human gene for oncomodulin is located on chromosome 7. It is a parvalbumin- family calcium-binding protein expressed and secreted by macrophages that typically traffic to tissue as an inflammatory response or after injury. Oncomodulin promotes axon regeneration in retinal ganglion cells and maintains functioning in mouse cochlear hair cells. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human Oncomodulin. Sensitivity: <10pg/ml Range: 31.2pg/ml-2000pg/ml Specificity: Recognizes natural and recombinant Human Oncomodulin. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human Oncomodulin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for Oncomodulin has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: S2-S109) and test samples are added to the wells, a biotinylated detection specific for Oncomodulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Oncomodulin amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human OCM antibody, 1x plate Lyophilized recombinant Human OCM standard, 2x2ng/tube Biotinylated anti-Human OCM antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
