| Thyroxine-binding globulin (TBG), also known as SERPINA7, is the major thyroid hormone transport protein in serum. There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3-triiodothyronine (T3) in the bloodstream. This gene belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined. Using DNA blot hybridization, it is detected that there is a single-copy TBG gene located on the long arm of the X chromosome. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human Serpin A7. Sensitivity: <10pg/ml Range: 31.2pg/ml-2000pg/ml Specificity: Recognizes natural and recombinant Human Serpin A7. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human Serpin A7 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Serpin A7 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A21-A415) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Serpin A7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Serpin A7 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human SERPINA7 antibody, 1x plate Lyophilized recombinant Human SERPINA7 standard, 2x2ng/tube Biotinylated anti-Human SERPINA7 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
