Sialic acid-binding Ig-like lectin 7, also known as CD328 (cluster of differentiation 328) or AIRM1, is a protein that in humans is encoded by the SIGLEC7 gene. And this gene is mapped to chromosome 19. AIRM1 is a novel member of the sialoadhesin family characterized by 3 immunoglobulin-like extracellular domains (1 N-terminal V type and 2 C2 type) and a classic immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic portion. The highest aa sequence similarity found was with the myeloid-specific CD33 molecule, and similar to other sialoadhesin molecules, AIRM1 appears to mediate sialic acid-dependent ligand recognition. Sample Type: Cell culture supernates, serum, plasma (Heparin). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human Siglec-7/CD328. Sensitivity: <10pg/ml Range: 125pg/ml-8000pg/ml Specificity: Recognizes natural and recombinant Human Siglec-7/CD328. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human Siglec-7/CD328 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for Siglec-7/CD328 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q19-L353) and test samples are added to the wells, a biotinylated detection specific for Siglec-7/CD328 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Siglec-7/CD328 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human SIGLEC7 antibody, 1x plate Lyophilized recombinant Human SIGLEC7 standard, 2x10ng/tube Biotinylated anti-Human SIGLEC7 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
