The thrombopoietin receptor (TPOR), also known as CD110, is a protein that in humans is encoded by the MPL (myeloproliferative leukemia virus) oncogene. The human MPL gene is mapped to chromosome 1p34. The protein encoded by the c-mpl gene, CD110, is a 635aa transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. Sample Type: Cell lysates, cell culture supernates, serum, plasma (EDTA or Heparin) Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Mouse TPOR/MPL. Sensitivity: <15pg/ml Range: 156pg/ml-10,000pg/ml Specificity: Recognizes natural and recombinant Mouse TPOR/MPL. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Mouse TPOR/MPL BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for TPOR/MPL has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q26-W482) and test samples are added to the wells, a biotinylated detection specific for TPOR/MPL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TPOR/MPL amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Mouse Mpl antibody, 1x plate Lyophilized recombinant Mouse Mpl standard, 2x10ng/tube Biotinylated anti-Mouse Mpl antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
