ULBP2 BioAssay(TM) ELISA Kit (Human) (UL16 binding protein 2)
Artikelnummer:
USB-396815
Hersteller Artikelnummer:
396815
Alternativnummer:
USB-396815-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Applikation:
ELISA
UL16 binding protein 2 (ULBP2) is a cell surface glycoprotein encoded by ULBP2 gene located on the chromosome 6. This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. Sample Type: Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human ULBP2. Sensitivity: <12pg/ml Range: 62.5pg/ml-4000pg/ml Specificity: Recognizes natural and recombinant Human ULBP2. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Human ULBP2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ULBP2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G26-S216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ULBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ULBP2 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Human ULBP2 antibody, 1x plate Lyophilized recombinant Human ULBP2 standard, 2x10ng/tube Biotinylated anti-Human ULBP2 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
