Dsbc, also known as Protein disulfide isomerase II, belong to the Dsb family. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones. DsbC consists of 236aa containing signal peptide (1-20aa). Recombinant E. coli Dsbc, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa21-236 of E. coli Dsbc, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~23.5kD (217aa), Confirmed by MALDI-TOF Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSQAEFD KAAEEVKRLK TQPTDEEMLF IYSHFKQATV GDVNTDRPGL LDLKGKAKWD SWNKLKGTSK ESAMKTYVEK VDELKKKYGI Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
