IL1R2, also known as interleukin-1 receptor type 2 isoform 1, is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein is expressed predominantly by, T cells, fibroblasts and endothelial cells. It binds interleukin-1alpha (IL1A), interleukin-1beta (IL1B), and interleukin 1 receptor antagonist (IL1RN), preventing them from binding to their regular receptors and thereby inhibiting the transduction of their signaling. Also, Interleukin-4 (IL4) is reported to antagonize the activity of interleukin-1 by inducing the expression and release of this cytokine. Recombinant human IL1R2, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa14-343 of human IL1R2, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~38.8kD (338aa), ~40-57kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: FTLQPAAHTG AARSCRFRGR HYKREFRLEG EPVALRCPQV PYWLWASVSP RINLTWHKND SARTVPGEEE TRMWAQDGAL WLLPALQEDS GTYVCTTRNA SYCDKMSIEL RVFENTDAFL PFISYPQILT LSTSGVLVCP DLSEFTRDKT DVKIQWYKDS LLLDKDNEKF LSVRGTTHLL VHDVALEDAG YYRCVLTFAH EGQQYNITRS IELRIKKKKE ETIPVIISPL KTISASLGSR LTIPCKVFLG TGTPLTTMLW WTANDTHIES AYPGGRVTEG PRQEYSENNE NYIEVPLIFD PVTREDLHMD FKCVVHNTLS FQTLRTTVKE LEHHHHHH Biological Activity: Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal to 400ng/ml with IL-1beta. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
