LCP2, also known as lymphocyte cytosolic protein 2, is an intracellular docking protein with a single SH2 recognition domain. It is involved in T-cell antigen receptor mediated signaling. This protein is phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. Recombinant human LCP2, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-533 of human LCP2, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~61.2kD (542aa), ~70-100kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPMALRNVP FRSEVLGWDP DSLADYFKKL NYKDCEKAVK KYHIDGARFL NLTENDIQKF PKLRVPILSK LSQEINKNEE RRSIFTRKPQ VPRFPEETES HEEDNGGWSS FEEDDYESPN DDQDGEDDGD YESPNEEEEA PVEDDADYEP PPSNDEEALQ NSILPAKPFP NSNSMYIDRP PSGKTPQQPP VPPQRPMAAL PPPPAGRNHS PLPPPQTNHE EPSRSRNHKT AKLPAPSIDR STKPPLDRSL APFDREPFTL GKKPPFSDKP SIPAGRSLGE HLPKIQKPPL PPTTERHERS SPLPGKKPPV PKHGWGPDRR ENDEDDVHQR PLPQPALLPM SSNTFPSRST KPSPMNPLPS SHMPGAFSES NSSFPQSASL PPYFSQGPSN RPPIRAEGRN FPLPLPNKPR PPSPAEEENS LNEEWYVSYI TRPEAEAALR KINQDGTFLV RDSSKKTTTN PYVLMVLYKD KVYNIQIRYQ KESQVYLLGT GLRGKEDFLS VSDIIDYFRK MPLLLIDGKN RGSRYQCTLT HAAGYPHHHH HH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
