MGAT2, also known as alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, is a single-pass type 2 membrane protein. It has the typical glycosyltransferase domains that composes a short N- terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. This protein catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. Sometimes mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. Recombinant human MGAT2, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa30-447 of human MGAT2, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~49.3kD (427aa), ~40-57kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPRQRKNEA LAPPLLDAEP ARGAGGRGGD HPSVAVGIRR VSNVSAASLV PAVPQPEADN LTLRYRSLVY QLNFDQTLRN VDKAGTWAPR ELVLVVQVHN RPEYLRLLLD SLRKAQGIDN VLVIFSHDFW STEINQLIAG VNFCPVLQVF FPFSIQLYPN EFPGSDPRDC PRDLPKNAAL KLGCINAEYP DSFGHYREAK FSQTKHHWWW KLHFVWERVK ILRDYAGLIL FLEEDHYLAP DFYHVFKKMW KLKQQECPEC DVLSLGTYSA SRSFYGMADK VDVKTWKSTE HNMGLALTRN AYQKLIECTD TFCTYDDYNW DWTLQYLTVS CLPKFWKVLV PQIPRIFHAG DCGMHHKKTC RPSTQSAQIE SLLNNNKQYM FPETLTISEK FTVVAISPPR KNGGWGDIRD HELCKSYRRL QHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
