PTPN11, also known as tyrosine-protein phosphatase non-receptor type 11 isoform 2, belongs to the protein-tyrosine phosphatase (PTP) family, non-receptor class 2 subfamily. It participates in signal transduction from the cell surface to the nucleus downstream of various receptor and cytosolic protein tyrosine kinases. This protein deficient cells have poor mobility because excessive phosphorylation of FAK and other proteins interferes with the rotation of cell attachment points in the focal adhesion complex. Recombinant human PTPN11, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa1-460 of human PTPN11, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~53.9kD (469aa), ~50-70kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) AA Sequence: ADPMTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCRHHHHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
