TNFRSF10D, also known as tumor necrosis factor receptor superfamily member 10D, is a member of the TNF-receptor superfamily. This protein contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplasmic death domain. Also, it is can protect cells bearing TRAIL R1 and/or TRAIL R2 from TRAIL-mediated apoptosis. Source: Recombinant protein corresponding to aa56-211 of human TNFRSF10D, fused to hIgG-His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~43.8kDa (395aa), ~40-57kD (SDS-PAGE under reducing conditions) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: ATIPRQDEVP QQTVAPQQQR RSLKEEECPA GSHRSEYTGA CNPCTEGVDY TIASNNLPSC LLCTVCKSGQ TNKSSCTTTR DTVCQCEKGS FQDKNSPEMC RTCRTGCPRG MVKVSNCTPR SDIKCKNESA ASSTGKTPAA EETVTTILGM LASPYHVEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH Biological Activity: Measured in a neutralizing assay using Jurkat human T lymphocyte. The ED50 for this effect is less or equal to 10ng/ml in the presence of 2ng/ml TRAIL. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
