POLR2A (DNA-Directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-Directed RNA Polymerase II Subunit A, DNA-Directed RNA Polymerase III Largest Subunit, RNA-Directed RNA Polymerase II Subunit RPB1, POLR2), Rab
POLR2A (DNA-Directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-Directed RNA Polymerase II Subunit A, DNA-Directed RNA Polymerase III Largest Subunit, RNA-Directed RNA Polymerase II Subunit RPB1, POLR2), Rab
POLR2A (DNA-Directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-Directed RNA Polymerase II Subunit A, DNA-Directed RNA Polymerase III Largest Subunit, RNA-Directed RNA Polymerase II Subunit RPB1, POLR2), Rab
Artikelnummer:
USB-397521
Hersteller Artikelnummer:
397521
Alternativnummer:
USB-397521-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
ELISA, IHC
Immunogen:
Recombinant protein corresponding to human POLR2A, (Position: A1148-H1384), expressed in E. coli.
DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.1-0.5ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
