Chlamydia trachomatis grade 2 antigen contains a high concentration of elementary bodies and chlamydial components in PBS. Chlamydia trachomatis is a non-motile gram negative intracellular bacterium, which has two forms during replication. The extracellular form, used in antigen preparations, is the elementary body. It has a diameter of 0.25 - 0.35 µm. The intracellular form is the reticulate or initial body which is 0.25 - 1 µm in diameter. Although Chlamydia trachomatis is antigenically complex only a few antigens are important in diagnostic testing. Sixty percent of the dry weight of the outer membrane of the elementary body is composed of major outer membrane protein (MOMP). MOMP is a transmembrane protein with type, species and genus reactive epitopes. It possesses an epitope used to characterize C. trachomatis into 15 serotypes or serovars. The outer membrane of C. trachomatis has a heat stable lipopolysaccharide antigen (LPS) with 2-keto-3-deoxyoctonic acid as an immunodominant =component. This antigen contains the reactive epitopes employed in genus specific serological tests. There is structural similarity and antigenic cross reactivity between chlamydial and other gram negative LPS moieties. Source: Chlamydia trachomatis, strain Chlamydia trachomatis LGV type II strain 434, from Mouse L cells Applications: Suitable for use in both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases and M capture formats. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: This antigen preparation is inactivated using gamma radiation. This procedure is effective primarily by damaging chlamydial genetic material. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Mouse L cells are infected with C. trachomatis elementary bodies. Optimally infected cells are harvested and disrupted by sonication in PBS. Cellular debris is removed using centrifugation.
Formulierung:
Supplied as a liquid in PBS, pH 7.2.
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