This preparation contains more than 90% virus specific protein. The predominant form of the antigen is whole virions suspended in NTE with sucrose. The preparation is free of detergent. Residual non-viral protein comes from host tissue and culture medium. Source: Highly purified (>99%) 125kD protein from P3H3 cells chronically infected with EBV. Applications: Suitable for use in a variety of immunoassay formats including micro well ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Detergent inactivation by lysis. Detergent treatment of P3H3 cells disrupts cellular and viral membranes and consequently renders the material non-infectious. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
~99%. Chronically infected cells are harvested by centrifugation and disrupted by sonication and cell debris is removed by centrifugation. This lysate is purified on an immunoaffinity column using a monoclonal antibody against gp125.
Formulierung:
Supplied as a liquid in 20mM Tris-HCl, pH 7.4, 3M MgCl2.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
