HPX, also known as hemopexin, is a plasma glycoprotein that belongs to the family of the acute- phase proteins whose synthesis is induced after an inflammatory event. This protein associates with HDL and influence the inflammatory properties of HDL. In addition it can also be said that HDL from HX-null mice is pro- inflammatory. Also, this protein binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation and is expressed in the central nervous system, in the retina, and in peripheral nerves has also been observed. Recombinant human HPX, fused to His-Tag at C-terminal, was expressed in Insect Cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa24-462 from human HPX, fused to His-Tag at C-terminal, was expressed in Insect Cell. Molecular Weight: ~50.4kD (448aa) ~50-70kD (SDS-PAGE under reducing conditions.) AA Sequence: ADPTPLPPTS AHGNVAEGET KPDPDVTERC SDGWSFDATT LDDNGTMLFF KGEFVWKSHK WDRELISERW KNFPSPVDAA FRQGHNSVFL IKGDKVWVYP PEKKEKGYPK LLQDEFPGIP SPLDAAVECH RGECQAEGVL FFQGDREWFW DLATGTMKER SWPAVGNCSS ALRWLGRYYC FQGNQFLRFD PVRGEVPPRY PRDVRDYFMP CPGRGHGHRN GTGHGNSTHH GPEYMRCSPH LVLSALTSDN HGATYAFSGT HYWRLDTSRD GWHSWPIAHQ WPQGPSAVDA AFSWEEKLYL VQGTQVYVFL TKGGYTLVSG YPKRLEKEVG TPHGIILDSV DAAFICPGSS RLHIMAGRRL WWLDLKSGAQ ATWTELPWPH EKVDGALCME KSLGPNSCSA NGPGLYLIHG PNLYCYSDVE KLNAAKALPQ PQNVTSLLGC THHHHHHH Endotoxin: <1EU/1ug (LAL) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
