JAM2, also known as junctional adhesion molecule B isoform 1, is a type 1 transmembrane receptor belonging to the immunoglobulin superfamily. This protein is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-delived peripheral blood dendritic cells. Also, it plays a role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. Recombinant human JAM2, fused to hIgG-His-tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa29-238 from human JAM2, fused to hIgG-His-tag at C-terminal, expressed in Insect Cell. Molecular Weight: ~50.7kD (158aa) ~50-70kD (SDS-PAGE under reducing conditions) AA Sequence: ADPFSAPKDQ QVVTAVEYQE AILACKTPKK TVSSRLEWKK LGRSVSFVYY QQTLQGDFKN RAEMIDFNIR IKNVTRSDAG KYRCEVSAPS EQGQNLEEDT VTLEVLVAPA VPSCEVPSSA LSGTVVELRC QDKEGNPAPE YTWFKDGIRL LENPRLGSQS TNSSYTMNTK TGTLQFNTVS KLDTGEYSCE ARNSVGYRRC PGKRMQVDDL NISLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH Endotoxin: <1EU/1ug (LAL) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
