The Lck BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Lck protein expresssion profile in cells. It can be used for measuring the relative amounts of Lck in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on Lck. Sensitivity: >5000cells/well Kit Components: 472493A: Microtiter Strips, 1x96 wells 472493B: TBS, 10X, 1x24ml 472493C: Quenching Buffer, 1x24ml 472493D: Blocking Buffer, 1x50ml 472493E: Wash Buffer, 15X, 1x50ml *472493F: Lck Pab, 100X, 1x60ul *472493G: GAPDH Mab, 100X, 1x60ul 472493H: IgG Pab (HRP), 1x6ml 472493J: IgG Mab (HRP), 1x6ml 472493K: Primary Antibody Diluent, 1x12ml 472493L: Substrate, 1x12ml 472493M: Stop Solution (2N Sulfuric Acid), 1x12ml 472493N: Crystal Violet Solution, 1x6ml *472493P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *472493F and *472493G are stable for 2 weeks at 4C.. *472493P should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
