The MtSSB Colorimetric Cell-Based ELISA is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MtSSB protein expression profile in cells. It can be used for measuring the relative amounts of MtSSB in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on MtSSB. Sensitivity: >5000cells/well Kit Components: 472626A: Microtiter Strips, 1x96 wells 472626B: TBS, 10X, 1x24ml 472626C: Quenching Buffer, 1x24ml 472626D: Blocking Buffer, 1x50ml 472626E: Wash Buffer, 15X, 1x50ml *472626F: MtSSB Pab, 100X, 1x60ul *472626G: GAPDH Mab, 100X, 1x60ul 472626H: IgG Pab (HRP), 1x6ml 472626J: IgG Mab (HRP), 1x6ml 472626K: Primary Antibody Diluent, 1x12ml 472626L: Substrate, 1x12ml 472626M: Stop Solution (2N Sulfuric Acid), 1x12ml 472626N: Crystal Violet Solution, 1x6ml *472626P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *472626F and *472626G are stable for 2 weeks at 4C.. *472626P should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
