The Phospho-ACK1 (Tyr284) BioAssay(TM) ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can monitor ACK1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated ACK1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ACK1 phosphorylation. Sensitivity: >5000cells/well Kit Components: 472789A: Microtiter Strips, 2x96 wells 472789B: TBS, 10X, 1x24ml 472789C: Quenching Buffer, 1x24ml 472789D: Blocking Buffer, 1x50ml 472789E: Wash Buffer, 15X, 1x50ml *472789F: Phospho-ACK1 (Tyr284) Pab, 100X, 1x60ul *472789G: ACK1 Pab, 100X, 1x60ul 472789H: GAPDH Mab, 100X, 1x60ul 472789J: IgG Pab (HRP), 1x12ml 472789K: IgG Mab (HRP), 1x12ml 472789L: Primary Antibody Diluent, 1x12ml 472789M: Substrate, 1x12ml 472789N: Stop Solution (2N Sulfuric Acid), 1x12ml 472789P: Crystal Violet Solution, 1x12ml *472789Q: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *472789F, *472789G and *472789H are stable for 2 weeks at 4C. *472789Q should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
