The Phospho-Lck (Tyr393) BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Lck protein phosphorylation and expression profile in cells. It can be used for measuring the relative amounts of Lck in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on Lck phosphorylation. Sensitivity: >5000cells/well Kit Components: 473190A: Microtiter Strips, 2x96 wells 473190B: TBS, 10X, 1x24ml 473190C: Quenching Buffer, 1x24ml 473190D: Blocking Buffer, 1x50ml 473190E: Wash Buffer, 15X, 1x50ml *473190F: Lck (Phospho-Tyr393) Pab, 100X, 1x60ul *473190G: Lck Pab, 100X. 1x60ul *473190H: GAPDH Mab, 100X, 1x60ul 473190J: IgG Pab (HRP), 1x12ml 473190K: IgG Mab (HRP), 1x12ml 473190L: Primary Antibody Diluent, 1x12ml 473190M: Substrate, 1x12ml 473190N: Stop Solution (2N Sulfuric Acid), 1x12ml 473190P: Crystal Violet Solution, 1x12ml *473190Q: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *473190F, *473190G and *473190H are stable for 2 weeks at 4C. *473190Q should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
