The Phospho-LIMK1/2 (Thr508) BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor LIMK1/2 protein phosphorylation and expression profile in cells. It can be used for measuring the relative amounts of phosphorylated LIMK1/2 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on LIMK1/2 phosphorylation. Sensitivity: >5000cells/well Kit Components: 473193A: Microtiter Strips, 2x96 wells 473193B: TBS, 10X, 1x24ml 473193C: Quenching Buffer, 1x24ml 473193D: Blocking Buffer, 1x50ml 473193E: Wash Buffer, 15X, 1x50ml *473193F: LIMK1/2 (Phospho-Thr508) Pab, 100X, 1x60ul *473193G: LIMK1/2 Pab, 100X. 1x60ul *473193H: GAPDH Mab, 100X, 1x60ul 473193J: IgG Pab (HRP), 1x12ml 473193K: IgG Mab (HRP), 1x12ml 473193L: Primary Antibody Diluent, 1x12ml 473193M: Substrate, 1x12ml 473193N: Stop Solution (2N Sulfuric Acid), 1x12ml 473193P: Crystal Violet Solution, 1x12ml *473193Q: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *473193F, *473193G and *473193H are stable for 2 weeks at 4C. *473193Q should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
