The Phospho-NPM (Thr199) BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NPM protein phosphorylation and expresssion profile in cells. It can be used for measuring the relative amounts of phosphorylated NPM in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on NPM phosphorylation. Sensitivity: >5000cells/well Kit Components: 473272A: Microtiter Strips, 2x96 wells 473272B: TBS, 10X, 1x24ml 473272C: Quenching Buffer, 1x24ml 473272D: Blocking Buffer, 1x50ml 473272E: Wash Buffer, 15X, 1x50ml *473272F: NPM (Thr199) Pab, 100X, 1x60ul *473272G: NPM, 100X, 1x60ul 473272H: GAPDH Mab, 100X, 1x60ul 473272J: IgG Pab (HRP), 1x12ml 473272K: IgG Mab (HRP), 1x12ml 473272L: Primary Antibody Diluent, 1x12ml 473272M: Substrate, 1x12ml 473272N: Stop Solution (2N Sulfuric Acid), 1x12ml 473272P: Crystal Violet Solution, 1x12ml *473272Q: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *473272F, *473272G and *473272H are stable for 2 weeks at 4C. *473272Q should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
