The Phospho-ZAP-70 (Tyr493) BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ZAP- 70 protein phosphorylation and expression profile in cells. It can be used for measuring the relative amounts of ZAP-70 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on ZAP-70 phosphorylation. Sensitivity: >5000cells/well Kit Components: 473529A: Microtiter Strips, 2x96 wells 473529B: TBS, 10X, 1x24ml 473529C: Quenching Buffer, 1x24ml 473529D: Blocking Buffer, 1x50ml 473529E: Wash Buffer, 15X, 1x50ml *473529F: ZAP-70 (Phospho-Tyr493) Pab, 100X, 1x60ul *473529G: ZAP-70 Pab, 100X. 1x60ul *473529H: GAPDH Mab, 100X, 1x60ul 473529J: IgG Pab (HRP), 1x12ml 473529K: IgG Mab (HRP), 1x12ml 473529L: Primary Antibody Diluent, 1x12ml 473529M: Substrate, 1x12ml 473529N: Stop Solution (2N Sulfuric Acid), 1x12ml 473529P: Crystal Violet Solution, 1x12ml *473529Q: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *473529F, *473529G and *473529H are stable for 2 weeks at 4C. *473529Q should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
