The UBF1 BioAssay(TM) ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor UBF1 protein expression profile in cells. It can be used for measuring the relative amounts of UBF1 in cultured cells as well as screening for the effects that various treatments, inhibitors (i.e. siRNA, or chemicals) or activators have on UBF1. Sensitivity: >5000cells/well Kit Components: 473875A: Microtiter Strips, 1x96 wells 473875B: TBS, 10X, 1x24ml *473875C: Quenching Buffer, 1x24ml 473875D: Blocking Buffer, 1x50ml 473875E: Wash Buffer, 15X, 1x50ml *473875F: UBF1 Pab, 100X, 1x60ul *473875G: GAPDH Mab, 100X, 1x60ul 473875H: IgG Pab (HRP), 1x6ml 473875J: IgG Mab (HRP), 1x6ml 473875K: Primary Antibody Diluent, 1x12ml 473875L: Substrate, 1x12ml 473875M: Stop Solution (2N Sulfuric Acid), 1x12ml 473875N: Crystal Violet Solution, 1x6ml *473875P: SDS Solution, 1x24ml Storage and Stability: Store all components at 4C. Reconstituted *473875F and *473875G are stable for 2 weeks at 4C. *473875C and *473875P should be stored at RT or warmed up to RT if stored at 4C. Other components are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
