FAM-FLICA Caspase 1 BioAssay(TM) Kit

Produktübersicht

• Artikelname: FAM-FLICA Caspase 1 BioAssay(TM) Kit
• Artikelnummer: USB-474008
• Hersteller Artikelnummer: 474008
• Alternativnummer: USB-474008-25,USB-474008-100
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The FAM FLICA Caspase-1 BioAssay(TM) Kit is an in vitro assay employs the fluorescent inhibitor probe FAM-YVAD-FMK to label active caspase-1 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry. Test Principle: Caspases play important roles in apoptosis and inflammation. This FLICA BioAssay(TM) kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-1 probe allows researchers to assess caspase-1 activation. The FLICA reagent FAM-YVAD-FMK enters each cell and irreversibly binds to activated caspase-1. Because the FAM-YVAD-FMK FLICA reagent becomes covalently coupled to t1x14x1 vialshe active enzyme, it is retained within the cell, while any unbound FAM-YVAD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining green fluorescent signal is a direct measure of the active caspase-1 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the BioAssay(TM) kit. Unfixed samples may also be analyzed with Propidium Iodide or Hoechst 33342 to detect necrosis or changes in nuclear morphology, respectively. Excitation/Emission: 488-492nm/515-535nm Kit Components: *474008A: FLICA Caspase Inhibitor Reagent, 1x1 vial or 4x1 vials 474008B: Apoptosis Wash Buffer, 10X, 1x15ml or 1x60ml 474008C: Fixative, 1x6ml 474008D: Propidium Iodide, 250ug/ml, 1x1ml 474008E: Hoechst 33342, 200ug/ml, 1x1ml Storage and Stability: Store *474008A at 4C. After reconstitution store at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.