FLICA 660 Caspase 3/7 BioAssay(TM) Kit

Produktübersicht

• Artikelname: FLICA 660 Caspase 3/7 BioAssay(TM) Kit
• Artikelnummer: USB-474020
• Hersteller Artikelnummer: 474020
• Alternativnummer: USB-474020-1
• Hersteller: US Biological
• Kategorie: Kits/Assays
• Applikation: FC

Produktbeschreibung

Sample Type: Cell culture Reagent Name: 660-DEVD-FMK Target: Caspase 3 and 7 Excitation/Emission: 660nm/690nm Method of Analysis: Flow Cytometry, Fluorescence Microscope Intended Use: Detect caspase 3 and 7 activity with the FLICA 660 Caspase 3/7 BioAssay(TM) Kit. This in vitro assay employs the fluorescent inhibitor probe 660-DEVD-FMK to label active caspase 3 and 7 enzyme in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry. Test Principle: Caspases play important roles in apoptosis and inflammation. FLICA BioAssay(TM) kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells. The FLICA 660 Caspase 3/7 probe allows researchers to assess caspase 3 & 7 activation. The FLICA reagent 660-DEVD-FMK enters each cell and irreversibly binds to activated caspase 3 and 7. Because the 660-DEVD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound 660-DEVD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining far red fluorescent signal is a direct measure of the active caspase 3 and 7 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by fluorescence microscopy or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative. Unfixed samples may also be analyzed with Hoechst stain to detect changes in nuclear morphology. Kit Components: FLICA Caspase-3/7 Inhibitor Reagent (660-DEVD-FMK), 1 vial 10X Apoptosis Wash Buffer, 1x15mL Fixative, 1x6mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.