SR-FLICA Caspase 9 BioAssay(TM) Kit

Produktübersicht

• Artikelname: SR-FLICA Caspase 9 BioAssay(TM) Kit
• Artikelnummer: USB-474044
• Hersteller Artikelnummer: 474044
• Alternativnummer: USB-474044-25,USB-474044-100
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Intended Use: Detect caspase-9 activity with the SR-FLICA Caspase-9 BioAssay(TM) Kit. This in vitro assay employs the fluorescent inhibitor probe SR-LEHD-FMK to label active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry. Test Principle: Caspases play important roles in apoptosis and inflammation. FLICA BioAssay(TM) kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FLICA reagent SR-LEHD-FMK enters each cell and irreversibly binds to activated caspase-9. Because the SR-LEHD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound SR-LEHD-FMK FLICA reagent diffuses out of the cell and is washed away. The remaining red fluorescent signal is a direct measure of the active caspase-9 enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound FLICA can be analyzed by a fluorescence plate reader, fluorescence microscopy, or flow cytometry. Cells labeled with the FLICA reagent may be read immediately or preserved for 16 hours using the fixative included in the BioAssay(TM) kit. Unfixed samples may also be analyzed with Hoechst 33342 to detect changes in nuclear morphology. Excitation/Emission: 550-580nm (peak 570nm)/ 590-600nm (peak 590nm Hoechst 33342: 364/480nm Kit Components: *474044A: FLICA Caspase-9 Reagent (SR-LEHD-FMK), 1x1vial or 1x4vials 474044B: Apoptosis Wash Buffer, 10X 1x15ml or 1x60ml 474044C: Fixative, 1x6ml 474044D: Hoechst 33342, 1x1ml Storage and Stability: Store *474044A powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.