Total Cytotoxicity Test BioAssay(TM) Kit

Produktübersicht

• Artikelname: Total Cytotoxicity Test BioAssay(TM) Kit
• Artikelnummer: USB-474046
• Hersteller Artikelnummer: 474046
• Alternativnummer: USB-474046-125,USB-474046-250
• Hersteller: US Biological
• Kategorie: Kits/Assays
• Applikation: FC

Produktbeschreibung

Sample Type: Cell culture Reagent Name: 7-AAD, CFSE, SR-VAD-FMK Target: Cell-mediated cytotoxicity, poly caspases Excitation/Emission: 7-AAD-546nm/647nm, CFSE-492nm/520nm, SR-FLICA-565nm/600nm Method of Analysis: Flow cytometry Intended Use: Total Cytotoxicity & Apoptosis Assay is a single-tube, tri-color assay for quantitative assessment of cell-mediated cytolytic activity due to apoptosis and necrosis. The assay employs a green fluorescent cellular stain, CFSE, to label target cells, the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity assay samples, and the orange-red SR-FLICA reagent, SR-VAD-FMK, to measure caspase activity in the target cell population. Analyze your results using a flow cytometer. Test Principle: Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. Total Cytotoxicity BioAssay(TM) allows you to assess cytolytic activity in cell culture. The Total Cytotoxicity & Apoptosis Assay includes three fluorescent reagents: CFSE, 7-AAD, and SR-FLICA. CFSE, a green fluorescing cellular stain, is utilized to identify the target cell population. The unstained effector cells are added and incubated with the target cells. SR-FLICA reagent is incubated with cells to identify those containing active caspases. 7-AAD, a red fluorescing live/dead stain, is then added to stain all necrotic cells red by binding to the DNA of membrane-compromised cells. Through flow cytometry, quantify four populations of cells: live, early apoptotic, late apoptotic, and necrotic. Kit Components: 125 tests: SR-FLICA Reagent (SR-VAD-FMK), 1 vial CFSE target cell stain, 1 vial 7-AAD vital stain, 1 vial 10X Assay Buffer, 1x30mL 250 tests: SR-FLICA Reagent (SR-VAD-FMK), 2 vials CFSE target cell stain, 1 vial 7-AAD vital stain, 2 vials 10X Assay Buffer, 1x60mL Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.