Helicobacter pylori is a spiral-shaped, Gram-negative rod approximately 0.5x3.0um in size. The catalase-positive organism has 4-6 sheathed flagella attached to one pole which allow for motility. Helicobacter pylori lives in the stomach and duodenum (section of intestine just below stomach). It has a unique way of adapting in the harsh environment of the stomach. The inside of the stomach is bathed in about half a gallon of gastric juice every day. Gastric juice is composed of digestive enzymes and concentrated hydrochloric acid, which can readily tear apart the toughest food or microorganism. Bacteria, viruses, and yesterdays steak dinner are all consumed in this deadly bath of chemicals. It used to be thought that the stomach contained no bacteria and was actually sterile, but Helicobacter pylori changed that. The stomach is protected from its own gastric juice by a thick layer of mucus that covers the stomach lining. Helicobacter pylori takes advantage of this protection by living in the mucus lining. Once H. pylori is safely ensconced in the mucus, it is able to fight the stomach acid that does reach it with an enzyme it possesses called urease. Urease converts urea, of which there is an abundant supply in the stomach (from saliva and gastric juices), into bicarbonate and ammonia, which are strong bases. This creates a cloud of acid neutralizing chemicals around the H. pylori, protecting it from the acid in the stomach. The reaction of urea hydrolysis is important for diagnosis of H. pylori by the breath test. Source: H. pylori culture Applications: Suitable for use as a positive control in Lateral Flow assays and when spiked into stool samples. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Inactivation: Binary Ethylenimine + Formaldehyde Inactivation + Heat treatment at 60C for 30 minutes. Confirmed by absence of colonies after 72 hours. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Lysate
Formulierung:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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