Thrombopoietin, Recombinant, Human, aa22-353, His-Tag

Produktübersicht

• Artikelname: Thrombopoietin, Recombinant, Human, aa22-353, His-Tag
• Artikelnummer: USB-497084
• Hersteller Artikelnummer: 497084
• Alternativnummer: USB-497084-10,USB-497084-20
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Thrombopoietin, also known as megakaryocyte growth and development factor (MGDF), is a member of EPO/TPO family. It is produced in the liver by both parenchymal cells and sinusoidal endothelial cells, as well as in the kidney by proximal convoluted tubule cells. It acts as a key regulator of megakaryocytopoiesis and thrombopoiesis that leads to platelet production. Also, this protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Thrombopoietin, like EPO, plays a role in brain development. It promotes apoptosis of newly generated neurons, an effect counteracted by EPO and neurotrophins. Recombinant human Recombinant protein corresponding to aa22-353 from human Thrombopoietin, fused to His-Tag at C-terminal, expressed in HEK293 cells. Molecular Weight: ~36.8kD Biological Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range 10ng/ml. Amino Acid Sequence: SPAPP ACDLRVLSKL LRDSHVLHSR LSQCPEVHPL PTPVLLPAVD FSLGEWKTQM EETKAQDILG AVTLLLEGVM AARGQLGPTC LSSLLGQLSG QVRLLLGALQ SLLGTQLPPQ GRTTAHKDPN AIFLSFQHLL RGKVRFLMLV GGSTLCVRRA PPTTAVPSRT SLVLTLNELP NRTSGLLETN FTASARTTGS GLLKWQQGFR AKIPGLLNQT SRSLDQIPGY LNRIHELLNG TRGLFPGPSR RTLGAPDISS GTSDTGSLPP NLQPGYSPSP THPPTGQYTL FPLPPTLPTP VVQLHPLLPD PSAPTPTPTS PLLNTSYTHS QNLSQEG Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 36.8343
• NCBI: 000451
• UniProt: P40225
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/1ug (LAL)
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.