Ebola and Marburg viruses are both filamentous, enveloped, non-segmented, negative-stranded RNA viruses of the family Filoviridae in the order Mononegavirales (Curran and Kolakofsky, 1999, Sanchez et al., 2001). Ebola virus particles comprise seven structural proteins of which four (nucleoprotein (NP), VP35, VP30, and the RNA-dependent RNA polymerase (L)) are components of the ribonucleoprotein complex that is responsible for viral genome replication. Glycoprotein (GP), VP40, and VP24 are membrane-associated proteins and VP24 is believed to be involved in nucleocapsid formation. Ebola virus NP is the largest nucleoprotein of the non-segmented negative-stranded RNA viruses (739aa residues) and can be divided into a N-terminal hydrophobic region (approximately 350aa) and a C-terminal hydrophilic region (Watanabe et al., 2006). O glycosylation and sialylation of NP is required for its interaction with VP35, and may be important for viral genome replication. The Zaire Ebola virus NP RNA complex has been described at near-atomic resolution using C-terminal truncated protein (residues aa1-450) in a human cell line in which it sequestered cellular RNA and assembled it into a rigid helix indistinguishable from the viral nucleocapsid core (Sugita et al., 2018). Despite the fact that severe hemorrhagic fever caused by Ebola virus is associated with extremely high mortality rates in human and nonhuman primates, an effective vaccine or antiviral drug is still to be developed. This Ebola Virus Nucleoprotein (NP) (Sudan) has been developed to aid in the ongoing research efforts towards developing new vaccine candidates and in vitro diagnostics. Recombinant protein corresponding to the C-terminal of Ebola Virus Nucleoprotein, Sudan strain, fused to His-Tag, expressed in E. coli. Applications: Suitable for use in ELISA, Western Blot and Lateral Flow. Other applications not tested. Recommended Dilutions: Optimal dilution determined by the researcher. Molecular Weight: ~15kD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
15
Reinheit:
95% (SDS-PAGE)
Formulierung:
Supplied as a liquid in PBS, 7.2, 0.02% sodium azide.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
