SFRP3, Recombinant, Human (Secreted Frizzled Related Protein 3, sFRP-3, FRZB, Fritz, Frzb1) (BSA Free)
Artikelnummer:
USB-497269
Hersteller Artikelnummer:
497269
Alternativnummer:
USB-497269-25
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Secreted Frizzled Related Protein-3 (sFRP-3), also known as FRZB, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled receptors. sFRPs are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-4). Mature human sFRP-3 shares 93% aa sequence identity with mouse and rat sFRP-3. It neutralizes the bioactivity of Wnt-1, -5a, -9a, Xenopus Xwnt-8, and EGF (5-8). sFRP-3 regulates somitic myogenesis and osteoblast differentiation as well as morphogenesis of the cochlear epithelium, hair follicle, and cardiac atrioventricular cushion (6, 8-10). sFRP-3 also functions as a tumor suppressor in a variety of cancers including melanoma, medulloblastoma, soft tissue sarcoma, and androgen-independent prostate cancer. It inhibits epithelial-mesenchymal transition, tumor growth and invasion, and the activity of MMP-2 and MMP-9 (7, 11-13). In contrast, it can promote tumor growth and angiogenesis in renal cancer (14). Recombinant protein corresponding to Ala33-Asn325 human sFRP-3, expressed in NS0 cell. Biological Activity: Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280. The ED50 for this effect is 0.05-0.25ug/ml N-terminal Sequence Analysis: Ala33 Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
