ESM1, also known as endothelial cell-specific molecule 1 isoform a, is a secreted cysteine-rich dermatan sulfate proteoglycan. This protein primarily expressed by endothelial cells within the vascular capillary network in the alveolar walls of the lung and also at lower level in kidney. The expression of it is upregulated by TNF-alpha, IL-beta or lipopolysaccharide and down-regulated by IFN-gamma. It plays roles on angiogenesis, sprouting, and may have potent implications in lung endothelial cell-leukocyte interaction. Also, this protein has dermatan sulfate (DS) that is covalently attached to serine 137. It binds using DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing ESM1 has been shown to induce tumor formation, suggesting that it may be involved in the pathophysiology of tumor growth in vivo. Description: Recombinant protein corresponding to aa20-184 of human ESM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. Molecular Weight: ~19.2kD (174aa) 18-28kD (SDS-PAGE under reducing conditions) Endotoxin: <0.1EU/1ug (LAL) AA Sequence: ADLWSNNYAV DCPQHCDSSE CKSSPRCKRT VLDDCGCCRV CAAGRGETCY RTVSGMDGMK CGPGLRCQPS NGEDPFGEEF GICKDCPYGT FGMDCRETCN CQSGICDRGT GKCLKFPFFQ YSVTKSSNRF VSLTEHDMAS GDGNIVREEV VKENAAGSPV MRKWLNPRHH HHHH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
