KLK11, also known as kallikrein-11 isoform 1, is belongs to the peptidases S1 family and Kallikrein subfamily. It is expressed in many tissues including cerebellum, prostate, salivary glands, stomach, lung, thymus, small intestine, spleen, liver, and uterus. These expression appear to be regulated by steroid hormones in the breast carcinoma cell line BT-474. Based on it serum levels, this protein has been proposed that ovarian cancer cases can be distinguished from healthy controls, who display very low expression. Also, it that is activated by thermolysin is active against thioester substrates such as dichloroisocoumarin and aprotinin. Recombinant human KLK11, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. Description: Recombinant protein corresponding to aa19-250 of human KLK11, fused to His-tag at C-terminus, expressed in Baculovirus Molecular Weight: ~26.7kD (241aa), ~28-40kD (SDS-PAGE under reducing conditions) Endotoxin: <0.1EU/1ug (LAL) AA Sequence: ADLETRIIKG FECKPHSQPW QAALFEKTRL LCGATLIAPR WLLTAAHCLK PRYIVHLGQH NLQKEEGCEQ TRTATESFPH PGFNNSLPNK DHRNDIMLVK MASPVSITWA VRPLTLSSRC VTAGTSCLIS GWGSTSSPQL RLPHTLRCAN ITIIEHQKCE NAYPGNITDT MVCASVQEGG KDSCQGDSGG PLVCNQSLQG IISWGQDPCA ITRKPGVYTK VCKYVDWIQE TMKNNHHHHH H Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
