Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With Cas9 nuclease, customers can screen highly efficient gRNA using in vitro DNA cleavage assays. It could be a powerful tool for gene editing. Cas9 Nuclease is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes without nuclear localization signal (NLS). Applications: Screening for highly efficient and specific targeting gRNAs by in vitro DNA cleavage using Cas9 Nuclease from S. pyogenes. Highly purified Cas9 antigen could be used for specific antibody production. Supplied With: Reaction Buffer, 10X Supplied as a liquid in 100mM Tris-HCl, 300mM sodium chloride, pH 7, 0.1mM EDTA, 1mM DTT, 50% glycerol. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Formulierung:
Supplied as a liquid in 10mM Tris, pH 7.4, 300mM sodium chloride, 0.1mM EDTA, 1mM DTT, 50% glycerol.
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