Complement Factor H (CFH) BioAssay(TM) ELISA Kit (Human) (Wide Range)
Artikelnummer:
USB-517120
Hersteller Artikelnummer:
517120
Alternativnummer:
USB-517120-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Sample Type: Serum, plasma and other biological fluids Intended Use: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFH in human serum, plasma and other biological fluids. Sensitivity: 8.52ng/ml Range: 23.44-1500ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Complement Factor H (CFH). No significant cross-reactivity or interference between Wide-range Complement Factor H (CFH) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CFH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of CFH in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1*120ul Detection Reagent B, 1*120ul TMB Substrate, 1*9ml Wash Buffer (30X), 1*20ml Standard Diluent, 1*20ml Assay Diluent A, 1*12ml Assay Diluent B, 1*12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
