The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA5B in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Sensitivity: 0.133ng/ml Range: 0.312-20ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of Semaphorin 5B (SEMA5B). No significant cross-reactivity or interference between Semaphorin 5B (SEMA5B) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to SEMA5B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to SEMA5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of SEMA5B in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1*120ul Detection Reagent B, 1*120ul TMB Substrate, 1*9ml Wash Buffer (30X), 1*20ml Standard Diluent, 1*20ml Assay Diluent A, 1*12ml Assay Diluent B, 1*12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
