Von Willebrand Factor (vWF) BioAssay(TM) ELISA Kit (Rat)
Artikelnummer:
USB-517764
Hersteller Artikelnummer:
517764
Alternativnummer:
USB-517764-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Sample Type: Plasma Intended Use: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of vWF in rat plasma. Sensitivity: 4.99ng/ml Range: 12.35-1000ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF). No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to vWF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled vWF and unlabeled vWF (Standards or samples) with the pre-coated antibody specific to vWF. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of vWF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of vWF in the sample. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1 Detection Reagent B, 1*120ul Reagent Diluent, 1*300ul TMB Substrate, 1*9ml Wash Buffer (30X), 1*20ml Standard Diluent, 1*20ml Assay Diluent A, 1*12ml Assay Diluent B, 1*12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
