Concanavalin A (Jackbean) Lectin (Con A) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers. Source: Jackbean Purity: Affinity purified Appearance: Light orange, clear liquid Form: Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Concentration: 2mg/ml Blood Specificity: Relatively weak blood agglutinin Sugar Specificity: alpha-Mannose, alpha-Glucose Inhibitory Carbohydrate: alpha-Methylmannoside + alpha-Methylglucoside Fluorescence: Green Divalent Ions: Ca++, Mn++ Excitation: 495-500nm Maximumm Emission: 514-521nm Maximum Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Affinity purified
Formulierung:
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM calcium chloride, 0.5mM manganese chloride, 0.1% trehalose, 0.01% sodium azide, 5% glycerol . Labeled with Fluorescein isothiocyanate (FITC).
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