Dylight 488 can be used to visualize the binding pattern of Euonymus europaeus Lectin (EEL/EEA) in cellular imaging and flow cytometry. DyLight 488 is a green-fluorescent dye that works over a broad pH range (pH 4-9). It is brighter and more photostable than many other green-fluorescent dyes, including FITC. Source: Spindle tree Blood Specificity: B>O Carbohydrate Specificity: Galalpha, 3Gal, high mannose N-type glycans Inhibitory Carbohydrate: Lactose Divalent Ions: Ca++, Zn++ Excitation: 493nm Emission: 518nm Storage and Stability: 50ug/ml will agglutinate human A2 erythrocytes. Storage and Stability: Store at 4C. Do Not Freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Dylight conjugates are sensitive to light.
Formulierung:
Supplied as a liquid in 10mM HEPES, 150mM sodium chloride, 0.1mM Ions, 0.02% sodium azide. Labeled with DyLight488.
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