Phaseolus limensis agglutinin (LBA) is isolated from lima bean seeds and purified by fractionation. LBA is composed of 30,000 Da subunits, of which two are joined by a disulfide bond. Each subunit contains a cysteine residue which is required for both its carbohydrate- and metal-ion-binding activities. It has a carbohydrate specificity of terminal GalNAcalpha(1,3)Gal that elutes with N-acetylgalactosamine. LBA agglutinates blood group A erythrocytes. Source: Lima bean Blood Specificity: A1 > A2 >> B Sugar Specificity: GalNAc alpha(1,3) [Fuc alpha(1,2)] Gal Inhibitory Carbohydrate: N-Acetylgalactosamine Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
60
Reinheit:
Semi-Pure
Formulierung:
Supplied as a lyophilized powder.
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