Pisum sativum Lectin (PSA/PSL) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. Fluorescein conjugates display a high rate of photobleaching, signal is sensitive to pH changes, broad fluorescence emission spectrum, and fluorescence quenching on conjugation to biopolymers. Source: Pea Blood Specificity: Non-specific Sugar Specificity: alpha-Mannose, alpha-Glucose Inhibitory Carbohydrate: alpha-Methylmannoside + alpha-Methylglucoside Fluorescence: Green Divalent Ions: Ca++, Mn++ Excitation: 495nm Emission: 515nm Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 6months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Affinity purified
Formulierung:
Supplied as a liquid in 10mM HEPES, 150mM sodium chloride, 0.1mM Ions, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
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