Affinity purified Vicia villosa lectin or agglutinin (VVL/VVA) is a tetramer composed of four subunits. VVL consists of A and B subunits, with a dominant isolectin that appears to be B subunit-rich. A4 isolectin agglutinates specifically A1 erythrocytes. B4 isolectin does not agglutinate A, B, or O-erythrocytes, however, B4 binds the Tn antigen, a molecule expressed on the surface of most tumor cells. A2B2 and similar tetramer isolectins display properties characteristic of both A4 and B4 isolections. Cy3 can be used to visualize the binding pattern of Vicia villosa Lectin (VVL/VVA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling Source: Hairy vetch Sugar Specificity: N-Acetylgalactosamine Inhibitory Carbohydrate: N-Acetylgalactosamine Fluorescence: Dark red Divalent Ions: Ca++, Mn++ Excitation: 550nm Emission: 570nm Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. CY3 conjugates are sensitive to light.
Formulierung:
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.05mM CaCl, 0.05mM MnCl, 1% BSA, 0.02% sodium azide. Labeled with Cy3.
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