Horseradish peroxidase (HRP) is a 40kD protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Vicia villosa Lectin (VVL/VVA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics. Source: Hairy vetch Activity: 250ug/ml is usally required to agglutinate type A1 cells. 1ug/ml will agglutinate neuraminidase cells. Sugar Specificity: N-Acetylgalactosamine Inhibitory Carbohydrate: N-Acetylgalactosamine Divalent Ions: Ca++, Mn++ Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Formulierung:
Supplied as a liquid in 10mM HEPES, 150mM sodium chloride, 0.05mM CaCl, 0.05mM MnCl, 1% BSA. Labeled with Horseradish peroxidase (HRP).
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