Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II) BioAssay(TM) ELISA Kit (Human)

Produktübersicht

• Artikelname: Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II) BioAssay(TM) ELISA Kit (Human)
• Artikelnummer: USB-530049
• Hersteller Artikelnummer: 530049
• Alternativnummer: USB-530049-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II) BioAssay(TM) ELISA Kit is a sandwich ELISA for the in vitro quantitative determination of PIVKA-II concentrations in human serum, plasma and other biological fluids. Detection Range: 0.16-10ng/ml Sensitivity: 0.09ng/ml Precision: Intra-Assay %CV: <10% Inter-Assay %CV: <10% Specificity: Recognizes human PIVKA-II. No significant cross-reactivity or interference between human PIVKA-II and analogues was observed. Test Principle: This BioAssay(TM) ELISA kit uses Sandwich-ELISA as the method. The micro ELISA strips provided in this kit has been pre-coated with an antibody specific to human PIVKA-II. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for human PIVKA-II and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain human PIVKA-II, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm 2nm. The OD value is proportional to the concentration of human PIVKA-II. The concentration of human PIVKA-II in samples can be calculated by comparing the OD of the samples to the standard curve. Kit Components: *530049A: Microtiter Strips,1x96 wells, Pre-coated, ready to use *530049B: Standard, 2x1vial 530049C: Standard Diluent, 1x20ml *530049D: Biotin Detection, 100X, 1x120ul *530049E: HRP Conjugate, 100X, 1x120ul 530049F: Biotin Detection Diluent, 1x14ml 530049G: HRP Diluent, 1x14ml 530049H: Substrate, 1x10ml 530049K: Stop Solution, 1x10ml 530049L: Wash Buffer, 25X, 1x30ml Precaution: The Stop Solution (530049K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *530049A, *530049B, *530049D and *530049E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 90 minutes at 37C. 3. Aspirate and add 100ul prepared Biotin Working Solution. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared HRP Detection Working Solution. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate. Incubate 15 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.