Arginase 1/ARG1, Recombinant, Human, aa1-322, His-Tag

Produktübersicht

• Artikelname: Arginase 1/ARG1, Recombinant, Human, aa1-322, His-Tag
• Artikelnummer: USB-532258
• Hersteller Artikelnummer: 532258
• Alternativnummer: USB-532258-50,USB-532258-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Arginase is a manganese-containing enzyme which catalyzes the hydrolysis of arginine to ornithine and urea. It is the final enzyme of the urea cycle. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The type II isoform has been implicated in the regulation of the arginine/ornithine concentrations in the cell. It is located in mitochondria of several tissues in the body, with most abundance in the kidney and prostate Source: Recombinant protein corresponding to aa1-322 from human Arginase 1/ARG1, fused to His-Tag, expressed in E. coli. Molecular Weight: ~35.8kD (330aa), confirmed by MALDI-TOF Specific Activity: 150,000 pmol/min/ug Unit Defintion: The amount of enzyme that hydrolyze 1.0 pmole of arginine to urea per minute at pH 10.5 at 37C. Amino Acid Sequence: MSAKSRTIGI IGAPFSKGQP RGGVEEGPTV LRKAGLLEKL KEQECDVKDY GDLPFADIPN DSPFQIVKNP RSVGKASEQL AGKVAEVKKN GRISLVLGGD HSLAIGSISG HARVHPDLGV IWVDAHTDIN TPLTTTSGNL HGQPVSFLLK ELKGKIPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYIL KTLGIKYFSM TEVDRLGIGK VMEETLSYLL GRKKRPIHLS FDVDGLDPSF TPATGTPVVG GLTYREGLYI TEEIYKTGLL SGLDIMEVNP SLGKTPEEVT RTVNTAVAIT LACFGLAREG NHKPIDYLNP PKLEHHHHHH Applications: Suitable for use in Enzyme Activity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 35.8
• NCBI: 000036
• UniProt: P05089
• Reinheit: 85% (SDS-PAGE). Endotoxin: 1EU/ug (LAL).
• Formulierung: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2mM DTT, 100mM sodium chloride, 20% glycerol.