BLMH, Recombinant, Mouse, aa1-455, His-Tag

Produktübersicht

• Artikelname: BLMH, Recombinant, Mouse, aa1-455, His-Tag
• Artikelnummer: USB-532267
• Hersteller Artikelnummer: 532267
• Alternativnummer: USB-532267-10,USB-532267-50
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

BLMH, also known as bleomycin hydrolase, is a member of the papain superfamily of the cysteine protease and the peptidase C1 family. It is a cytoplasmic cysteinepeptidase commonly found as a homohexamer. The normal physiological role of BLMH is unknown, but it protects normal and malignant cells from the glycopeptide antitumor drug Blm. It catalyzes the inactivation of the antitumor drug Blm (a glycopeptide) by hydrolyzing the carboxyamide bond of its B-aminoalaninamide moiety and also shows general aminopeptidase activity. Source: Recombinant protein corresponding to aa1-455 from mouse BLMH fused to His-Tag at N-terminal expressed in E. coli. Molecular Weight: ~54.9kD (478aa), confirmed by MALDI-TOF Endotoxin: ~1EU/1ug (LAL) Biological Activity: Specific activity is > 1,500 pmole/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Met-AMC to Methionine and AMC per minute at pH7.5 at 37C. AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNNAGLN SEKVSALIQK LNSDPQFVLA QNVGTTHDLL DICLRRATVQ GAQHVFQHVV PQEGKPVTNQ KSSGRCWIFS CLNVMRLPFM KKFNIEEFEF SQSYLFFWDK VERCYFFLNA FVDTAQKKEP EDGRLVQYLL MNPTNDGGQW DMLVNIVEKY GVVPKKCFPE SHTTEATRRM NDILNHKMRE FCIRLRNLVH SGATKGEISS TQDAMMEEIF RVVCICLGNP PETFTWEYRD KDKNYHKIGP ITPLQFYKEH VKPLFNMEDK ICFVNDPRPQ HKYNKLYTVD YLSNMVGGRK TLYNNQPIDF LKKMVAASIK DGEAVWFGCD VGKHFNGKLG LSDMNVYDHE LVFGVSLKNM NKAERLAFGE SLMTHAMTFT AVSEKDNQEG TFVKWRVENS WGEDHGHKGY LCMTDEWFSE YVYEVVVDKK HVPEEVLAVL EQEPIVLPAW DPMGALAE Applications: Suitable for use in Enzyme Activity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 54.9
• NCBI: 848760
• UniProt: Q8R016
• Reinheit: 90% (SDS-PAGE)
• Formulierung: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 30% glycerol.