Caspase-3, Recombinant, Human, aa29-277, His-Tag

Produktübersicht

• Artikelname: Caspase-3, Recombinant, Human, aa29-277, His-Tag
• Artikelnummer: USB-532271
• Hersteller Artikelnummer: 532271
• Alternativnummer: USB-532271-10,USB-532271-50
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Caspase-3, also known as caspase-3 isoform a, is a member of the cysteine-aspartic acid protease (caspase) family of enzymes. This protein is most abundant in cell lines of lymphocytic origin. Also, it activates caspases 6 and 7 and the protein itself is processed and is activated by caspases 8, 9, and 10. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits that dimerize to form the active enzyme. Members of the caspase family of proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Recombinant protein corresponding to aa29-277 from human Caspase-3, fused to His-Tag at C-terminal, expressed in Baculovirus.. Molecular Weight: ~29.4kD (256aa) Specific Activity: > 5,000 pmol/min/ug. Unit Definition: One unit will liberate 1 pmoles of Ac-DEVD-AFC to Ac-DEVD and AFC per minute at pH7.5 at 25C. AA Sequence: MSGISLDNSY KMDYPEMGLC IIINNKNFHK STGMTSRSGT DVDAANLRET FRNLKYEVRN KNDLTREEIV ELMRDVSKED HSKRSSFVCV LLSHGEEGII FGTNGPVDLK KITNFFRGDR CRSLTGKPKL FIIQACRGTE LDCGIETDSG VDDDMACHKI PVEADFLYAY STAPGYYSWR NSKDGSWFIQ SLCAMLKQYA DKLEFMHILT RVNRKVATEF ESFSFDATFH AKKQIPCIVS MLTKELYFYH HHHHHH Applications: Suitable for use in Enzyme Activity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer,

Produkteigenschaften

• Molekulargewicht: 29.4
• NCBI: 116786
• UniProt: P42574
• Reinheit: 90% (SDS-PAGE). Endotoxin: 1EU/ug (LAL).
• Formulierung: Supplied as a liquid in 20mM HEPES, pH 7.5, 0.1M sodium chloride, 1mM EDTA, 1mM DTT, 20% glycerol.