Granzyme B, Recombinant, Mouse, aa19-247, His-Tag
Artikelnummer:
USB-532310
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| Artikelname: | Granzyme B, Recombinant, Mouse, aa19-247, His-Tag |
| Artikelnummer: | USB-532310 |
| Hersteller Artikelnummer: | 532310 |
| Alternativnummer: | USB-532310-50,USB-532310-100 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| Granzyme B, also known as Gzmb, is a member of the granzyme family of cell death-inducing serine proteases specially expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. It plays crucial role for target cell lysis in cell-mediated immune responses. This protein is linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. It cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Recombinant protein corresponding to aa19-247 from mouse Granzyme B fused to His-Tag at C-terminal, expressed in Baculovirus. Molecular Weight: ~26.3kD (235aa) Biological Activity: Specific activity is > 9,000 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C. Amino Acid Sequence: GEIIGGHEVK PHSRPYMALL SIKDQQPEAI CGGFLIREDF VLTAAHCEGS IINVTLGAHN IKEQEKTQQV IPMVKCIPHP DYNPKTFSND IMLLKLKSKA KRTRAVRPLN LPRRNVNVKP GDVCYVAGWG RMAPMGKYSN TLQEVELTVQ KDRECESYFK NRYNKTNQIC AGDPKTKRAS FRGDSGGPLV CKKVAAGIVS YGYKDGSPPR AFTKVSSFLS WIKKTMKSSH HHHHH Applications: Suitable for use in Enzyme Activity and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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