Luciferase, Recombinant, Firefly, aa1-550, His-Tag
Artikelnummer:
USB-532340
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| Artikelname: | Luciferase, Recombinant, Firefly, aa1-550, His-Tag |
| Artikelnummer: | USB-532340 |
| Hersteller Artikelnummer: | 532340 |
| Alternativnummer: | USB-532340-50,USB-532340-100,USB-532340-250,USB-532340-500 |
| Hersteller: | US Biological |
| Kategorie: | Molekularbiologie |
| Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Recombinant protein corresponding to aa1-550 from firefly Luciferase fused to His-Tag, at C-terminal, expressed in E. coli. Uniprot/Accession: A3KBZ5 Molecular Weight: ~63kD (571aa), confirmed by MALDI-TOF Specific Activity: 1x10e10 light units/mg. Unit Definition: One luciferase enzyme units will produce one Relative Light Unit (RLU) at 25C Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMEDAKNIKK GPAPFYPLED GTAGEQLHKA MKRYALVPGT IAFTDAHIEV NITYAEYFEM SVRLAEAMKR YGLNTNHRIV VCSENSLQFF MPVLGALFIG VAVAPANDIY NERELLNSMN ISQPTVVFVS KKGLQKILNV QKKLPIIQKI IIMDSKTDYQ GFQSMYTFVT SHLPPGFNEY DFVPESFDRD KTIALIMNSS GSTGLPKGVA LPHRTACVRF SHARDPIFGN QIIPDTAILS VVPFHHGFGM FTTLGYLICG FRVVLMYRFE EELFLRSLQD YKIQSALLVP TLFSFFAKST LIDKYDLSNL HEIASGGAPL SKEVGEAVAK RFHLPGIRQG YGLTETTSAI LITPEGDDKP GAVGKVVPFF EAKVVDLDTG KTLGVNQRGE LCVRGPMIMS GYVNNPEATN ALIDKDGWLH SGDLAYWDED EHFFIVDRLK SLIKYKGYQV APAELESILL QHPNIFDAGV AGLPDDDAGE LPAAVVVLEH GKTMTEKEIV DYVASQVTTA KKLRGGVVFV DEVPKGLTGK LDARKIREIL IKAKKGGKSK L Applications: Suitable for use in Enzyme Activity and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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